Application

Western blot: 1:500 to 1:2000.

Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-Apaf-1 Antibody, C-terminus detects level of Apaf-1 & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: C-terminus

Rabbit anti-Apaf1 (C-terminus) polyclonal antibody was raised against a peptide corresponding to amino acids 1158 to 1177 of human Apaf1 (Zou et al. 1997). The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid (Zou et al. 1997; Cecconi et al. 1998).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-579

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Specificity

Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and was designated Apaf1 (for apoptosis protease-activating factor 1; Zou et al. 1997; Cecconi et al. 1998). Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 which is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Li et al. 1997). Apaf1 can also associate with caspase-4 and caspase-8 (Hu et al. 1998). Apaf1 is ubiquitously expressed in human tissues (Zou et al. 1997).

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.